Convert Peak Table to Fragments_repeats class
peak_table_to_fragments.Rd
This function converts a peak table data frame into a list of fragments_repeats objects.
Usage
peak_table_to_fragments(
df,
data_format = NULL,
peak_size_col = NULL,
peak_signal_col = NULL,
unique_id = NULL,
dye_col = NULL,
dye_channel = NULL,
allele_col = NULL,
min_size_bp = 200,
max_size_bp = 1000
)
Arguments
- df
A data frame containing the peak data.
- data_format
The format that the data frame is in (for example, a genemapper peak table). Choose between: genemapper5, generic.
- peak_size_col
A character string specifying column name giving the peak size.
- peak_signal_col
A character string specifying column name giving the peak signal.
- unique_id
A character string specifying column name giving the unique sample id (often the file name).
- dye_col
Genemapper specific. A character string specifying column name indicating the dye channel.
- dye_channel
Genemapper specific. A character string indicating the channel to extract data from. For example, 6-FAM is often "B".
- allele_col
Genemapper specific. A character string specifying column name indicating the called alleles. This is often used when the peaks have been called in genemapper.
- min_size_bp
Numeric value indicating the minimum size of the peak table to import.
- max_size_bp
Numeric value indicating the maximum size of the peak table to import.
Details
This function takes a peak table data frame (eg. Genemapper output) and converts it into a list of fragment objects. The function supports different data formats and allows specifying column names for various attributes.
Examples
gm_raw <- trace::example_data
test_fragments <- peak_table_to_fragments(
gm_raw,
data_format = "genemapper5",
dye_channel = "B",
min_size_bp = 400
)